![]() ![]() Plants exhibit various changes in phenotype under N starvation, including reduced seed production (yield), leaf chlorosis, stunted growth, modulation in root architecture, etc., underlining the importance of N to plant growth and development. amino acids, nucleotides, proteins, chlorophyll, and many plant hormones, it is considered the major essential nutrient required for plant growth and development. Nitrogen (N), being the constituent of most biomolecules, viz. Physical mapping of DEGs revealed 95 DEGs in roots and 76 in shoots to be present in quantitative trait loci (QTL) known for NUE. Most of these DEGs belonged to starch and chloroplast metabolism, followed by membrane and signaling proteins. Fifty-two of the 89 DEGs identified as specific to N22 root tissues were also found to be differentially expressed between the two genotypes under N−. There were a total of 385 nitrogen-responsive DEGs (106 in shoots and 279 in roots) between the two genotypes. The response of the two genotypes to N starvation was distinctly different reflecting their morphological/biochemical response with just two and eight common DEGs in the root and shoot tissues. Transcriptome analysis of these genotypes revealed nearly double the number of genes to be differentially expressed (DEGs) in roots (1016) compared to shoots (571). Glutamate synthase (GOGAT) and citrate synthase CS activity were highly reduced in N22 but not in IR64. Nitrate reductase showed significantly low specific activity under N− in both genotypes. Similarly, the photosynthetic pigments showed a drastic reduction in N22 under low N, while IR64 was more resilient. The two genotypes were found to be contrasting in their response to N− IR64 root architecture and root dry weight remained almost equivalent to that under N+ conditions, while N22 showed high foraging ability but a substantial reduction in biomass under N−. Here we report the genome-wide transcriptome analysis of two rice genotypes, IR 64 (IR64) and Nagina 22 (N22) under optimal (N+) and chronic starvation (N−) of nitrogen (N) from 15-day-old root and shoot tissues. The nitrogen use efficiency (NUE) of crop plants is limited and enhancing it in rice, a major cereal crop, would be beneficial for farmers and the environment alike. ![]()
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